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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAITRL. Human AITRL specific antibody was purified by affinity chromatography and then biotinylated. |
| Target | TNFSF18 |
| Conjugation | Biotin |
Storage & Handling
−| Storage | AITRL antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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To detect hAITRL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human AITRL (orb1272727) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hAITRL.

To detect hAITRL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hAITRL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
TNFSF18 Antibody (Biotin) (orb1272726)
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