AIMP1 Antibody

• Catalog Number: orb1248689
• Category: Antibodies
• Description: AIMP1 Antibody
• Target: AIMP1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-KQQSIAGSADSKP
• UniProt ID: Q12904
• MW: Approx 37 kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in Human Lymph Node and Uterus lysates (calculated MW of 37.0kDa according to NP_001135887.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Pancreas shows staining in the cytoplasm towards the alpha cells periphery. Recommended concentration, 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AIMP1, aminoacyl tRNA synthetase complex-interacti
• Note: For research use only
• NCBI: NP_001135888.1, NP_004748.2
• Expiration Date: 12 months from date of receipt.

orb1248689 (0.1 ug/ml) staining of Human Lymph Node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248689 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.