AIM2 Antibody

• Catalog Number: orb1249157
• Category: Antibodies
• Description: AIM2 Antibody
• Target: AIM2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-GEKLQLTSGVHSTIK
• UniProt ID: O14862
• Tested applications: ELISA, FC, IF, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 37kDa band observed in lysates of cell line HeLa (calculated MW of 39.0kDa according to Human NP_004824.1). Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6µg/ml. Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AIM2, absent in melanoma 2, PYHIN4
• Research Area: Cancer Biology, Immunology & Inflammation
• Note: For research use only
• NCBI: NP_004824.1

orb1249157 (1 ug/ml) staining of IFNbeta-treated WI-38 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249157 (3.8 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.