AIM2 Antibody (N-term)

SKU: orb1927673

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, FC - 1:10-50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This AIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human AIM2. Antigen Region: 4-32 aa.
• Target: AIM2 {ECO:0000303|PubMed:9242382, ECO:0000312|HGNC:HGNC:357}
• Molecular Weight: 38954 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interferon-inducible protein AIM2, Absent in melanoma 2, AIM2

Western blot analysis of AIM2 Antibody (N-term) in MCF-7, HL-60, K562, Hela cell line lysates (35 ug/lane). AIM2 (arrow) was detected using the purified Pab.

AIM2 Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of AIM2 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

AIM2 {ECO:0000303|PubMed:9242382, ECO:0000312|HGNC:HGNC:357}

UniProt

O14862

RefSeq (Protein):NP_004824.1