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Catalog Number | orb1938983 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Target | This AIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 amino acids from the N-terminal region of human AIF1. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
UniProt ID | P55008 |
MW | 16703 Da |
Tested applications | IHC-P, WB |
Dilution range | WB: 1:2000, WB: 1:2000, IHC-P: 1:50~100 |
Antibody Type | Primary Antibody |
Clone Number | RB14652 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Allograft inflammatory factor 1, AIF-1, Ionized ca Read more... |
Note | For research use only |
NCBI | NP_116573.1, NP_004838.1, NP_001614.3 |
AIF1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AIF1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti-AIF1 Antibody (N-term) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lane 3: THP-1 whole cell lysate. Lane 4: human spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-AIF1 Antibody (N-term) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lane 3: THP-1 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.