AHA1 Antibody

• Catalog Number: orb1822419
• Category: Antibodies
• Description: Rabbit Anti-Mouse AHA1 Polyclonal
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant Full Length Mouse Aha1 Protein
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:60); optimal dilutions for assays should be determined by the user.
• Conjugation: Unconjugated
• MW: 38kDa
• Target: AHA1
• Entrez: 217737
• UniProt ID: Q8BK64
• NCBI: NP_666148.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: Activator of 90 kDa heat shock protein ATPase homo
• Note: For research use only
• Application notes: 1 µl/ml of SPC-183 was sufficient for detection of Aha1 in 10 µg of mixed human cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA1 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA1 Polyclonal Antibody at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of multiple Cell line lysates showing detection of AHA1 protein using Rabbit Anti-AHA1 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AHA1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.