AGL Antibody (C-term)

SKU: orb1931627

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:8000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD6190
• Immunogen: This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1479-1510 amino acids from the C-terminal region of human AGL. Antigen Region: 1479-1510 aa.
• Target: AGL
• Molecular Weight: 174764 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1,4-1,4-glucantransferase, Amylo-alpha-1,6-glucosidase, Amylo-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, AGL, GDE

Confocal immunofluorescent analysis of AGL Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot using anti-AGL (C-term) antibody at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue.

Expression of myc-GS causes wild type but not the ÄCBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ÄCBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.

Anti-AGL Antibody (C-term) at 1: 8000 dilution + human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 175 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

AGL

UniProt

P35573

RefSeq (Protein):NP_000634.2, NP_000636.2, NP_000019.2, NP_000637.2, NP_000633.2, NP_000635.2