You have no items in your shopping cart.
Cart summary
Item 1 of 4
Item 1 of 4
AGL Antibody (C-term)
Catalog Number: orb1931627
| Catalog Number | orb1931627 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB4978 |
| Tested applications | IF, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, IF: 1:10~50, WB: 1:1000, WB: 1:8000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 174764 Da |
| Target | This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1479-1510 amino acids from the C-terminal region of human AGL. |
| UniProt ID | P35573 |
| NCBI | NP_000633.2, NP_000634.2, NP_000636.2, NP_000637.2, NP_000019.2, NP_000635.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Glycogen debranching enzyme, Glycogen debrancher, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of AGL Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot using anti-AGL (C-term) antibody at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue.
Expression of myc-GS causes wild type but not the ÃCBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ÃCBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.
Anti-AGL Antibody (C-term) at 1: 8000 dilution + human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 175 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
