AG1024

SKU: orb1305156

Description

AG1024

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 65678-07-1
• MW: 305.17
• Purity: 99.23%
• Formula: C₁₄H₁₃BrN₂O
• SMILES: CC(C)(C)c1cc(C=C(C#N)C#N)cc(Br)c1O
• Target: IGF-1R,Apoptosis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.55 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:57 mg/mL (186.78 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  IGF-1R:7 μM|Insulin receptor:57 μM
• In Vivo:
  AG-1024 effectively inhibits autophosphorylation of the IGF-1 receptor (IC50=7 μM) and IR (IC50=57 μM). In MCF-7 cells, at a concentration of 10 μM, AG-1024 downregulates phosphorylated Akt1 and bcl-2, while upregulating Bax, p53, and p21, thereby inhibiting cell proliferation.
• In Vitro:
  In mice carrying Ba/F3-p210 xenografts, the administration of AG-1024 (30 μg) for 10 days significantly inhibited tumor growth.
• Cell Research:
  Cells are exposed to AG-1024 for 24, 48 or 72 hours. For the determination of proliferation, cells are harvested and counted with trypan blue dye exclusion. Apoptosis is evaluated by dual staining of MCF-7 with fluoresceine anti-digoxigenin and propidium iodide. Briefly, fixed cells are washed with PBS, suspended in TdT buffer with TdT enzyme and Dig-dUTP for 60 minutes, and suspended in FITC blocking solution with anti-Dig-Fluorescein for 30 minutes at room temperature and kept in a dark place. Cells are then rinsed in buffer and resuspended in propidium iodide/RNase A solution for 30 minutes then analyzed by flow cytometry. For the assessment of phospho-Akt1, Bax, p53, bcl-2 and p21, cells are lysed and analyzed by western blot.(Only for Reference)

Storage & Handling

• Storage: store at low temperature,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IGF-1R, IGF1R, Insulin Receptor, factor-1, DNA-PKcs, AG 1024, AG1024, AG-1024, AGS 200, AGS200, AGS-200, BCR-ABL, Apoptosis, anti-cancer, Tyrphostin AG 1024, Tyrphostin AG1024, Tyrphostin AG-1024

Compound Identifiers

PubChem CID

2044