AG1024

SKU: orb1221911

Description

AG-1024 is also called Tyrphostin, is a selective inhibitor of IGF-1R. AG-1024 was used to evaluate effects on proliferation, radiosensitivity, and radiation-induced cell apoptosis in a human breast cancer cell line MCF-7. Exposure to Tyrphostin AG 1024 inhibited proliferation and induced apoptosis in a time-dependent manner, and the degree of growth inhibition for IC20 plus irradiation (4 Gy) was up to 50% compared to the control. Tyrphostin AG 1024 could be used as a potential therapeutic agent in combination with irradiation.

Key Properties

• CAS Number: 65678-07-1
• MW: 305.17
• Purity: >98% (HPLC)
• Formula: C₁₄H₁₃BrN₂O
• SMILES: CC(C)(C)c1c(c(cc(c1)C=C(C#N)C#N)Br)O
• Target: IGF-1R| Insulin Receptor
• Solubility: DMSO : ≥ 50 mg/mL; 163.84 mM

Bioactivity

• In Vivo:
  AG1024 (Tyrphostin AG 1024; 30 μg; i.p.; per day; for 2 weeks) significantly delays the tumour growth. Animal model: Female nude mice (6-8 weeks old). Dosage: 30 μg. Administration: IP; per day; for 2 weeks. Result: Significantly delayed the tumour growth.
• In Vitro:
  AG1024 (Tyrphostin AG 1024; 2-10 μM; 1-5 days) shows a dose-dependent inhibition of cell proliferation. AG1024 (1-5 μM; 1-3 days) induces UT7-9 and Baf3-p210 cells apoptosis. AG1024 (2 μM; 6, 12 hours) downregulates phospho-Akt, Bcr-Abl and upregulates DNA-PKcs. Cell Proliferation Assay Cell line: UT7-9 and Baf3-p210 cells. Concentration: 2, 5, 10 μM. Incubation time: 1, 3, 5 days. Result: Showed a dose-dependent inhibition of cell proliferation. Apoptosis Analysis Cell line: UT7-9 and Baf3-p210 cells. Concentration: 1, 3, 5 μM. Incubation time: 1, 2, 3 days. Result: Induced apoptosis. Western blot analysis. Cell line: UT7-9 and Ba/F3-p210 cells. Concentration: 2 μM. Incubation time: 6, 12 hours. Result: Downregulated phospho-Akt, Bcr-Abl and upregulated DNA-PKcs.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AG1024 | AG-1024 | AG 1024. Tyrphostin

Quick Database Links

Gene Symbol

IGF-1R| Insulin Receptor