Aftin-4

SKU: orb1223021

Description

Aftin-4, an amyloid forty-two inducer, activates γ-secretase, promoting the generation of amyloid-β-1-42 (Aβ1-42) from amyloid-β protein precursor. Aftin-4 increased Aβ-1-42, but not Aβ-1-40 in mouse hipppocampus, accompanied by learning deficits and mitochondrial ultrastructural changes similar to those found in the brains of patients with AD. Aftin-4 can thus be used to induce a rapid, acute Alzheimer's disease-like toxicity in the rodent brain.

Key Properties

• CAS Number: 866893-90-5
• MW: 368.48
• Purity: >98% (HPLC)
• Formula: C₂₀H₂₈N₆O
• SMILES: CC[C@H](CO)Nc2nc1c(ncn1C(C)C)c(n2)N(C)Cc3ccccc3
• Target: γ secretase(Aβ42)
• Solubility: DMSO : ≥ 250 mg/mL; 678.46 mM

Bioactivity

• In Vivo:
  Aftin-4 increases Aβ1-42 levels In vivo in mice and provokes rapidly a sustained toxicity highly reminiscent of Alzheimer’s disease (AD). Aftin-4 is administered at increasing doses, between 3 and 20 nmol/mouse, into the lateral ventricle and animals are sacrificed at various time points, between 3 to 14 days after injection. The hippocampus is dissected out the contents in Aβ1-40 or Aβ1-42 is determined using a mouse ELISA assay. Aftin-4 dose-dependently and significantly increases Aβ1-42 content, up to +216% at the highest dose tested.
• In Vitro:
  Aftin-4 selectively and potently increases Aβ1-42 in N2a cells, primary neurons, and brain lysates, with an EC50 value around 30 μM.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Aftin-4 | Aftin 4 | Aftin4

Quick Database Links

Gene Symbol

γ secretase(Aβ42)