AFP Antibody (N-term)

SKU: orb1939415

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:31.25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: B365EV72X33X0
• Immunogen: This AFP antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human AFP. Antigen Region: 56-85 aa.
• Target: AFP
• Molecular Weight: 68678 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP

Anti- at 1:1000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgM, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-AFP Antibody (N-term) at 1:31.25 dilution + MCF-7 whole cell lysate. Secondary Goat Anti-mouse IgM, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68678 Da. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

AFP

UniProt

P02771

RefSeq (Protein):NP_001125.1