AFG3L2 Antibody

• Catalog Number: orb1259526
• Category: Antibodies
• Description: AFG3L2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 538-797 of human AFG3L2 (NP_006787.2).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 89kDa
• Target: AFG3L2
• UniProt ID: Q9Y4W6
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: AFG3L2, FLJ25993, SCA28, SPAX5
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using AFG3L2 antibody (orb1259526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat brain using AFG3L2 antibody (orb1259526) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using AFG3L2 antibody (orb1259526) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using AFG3L2 antibody (orb1259526) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human appendix using AFG3L2 antibody (orb1259526) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using AFG3L2 antibody (orb1259526) at dilution of 1:200 (40x lens).