AEG 3482

SKU: orb2901495

Description

AEG3482 is a potent antiapoptotic compound that inhibits Jun kinase (JNK) activity through induced expression of heat shock protein 70 (HSP70). AEG3482 directly binds HSP90, thereby facilitating HSF1-dependent expression of HSP70 and HSP25.

Key Properties

• CAS Number: 63735-71-7
• MW: 280.33
• Purity: >98% (HPLC)
• Formula: C₁₀H₈N₄O₂S₂
• SMILES: NS(=O)(=O)c1nn2cc(nc2s1)-c1ccccc1
• Target: Apoptosis
• Solubility: In Vitro: DMSO : 250 mg/mL (891.81 mM; Ultrasonic )

Bioactivity

• In Vitro:
  AEG3482 (0.3-30 μM; 2 d) inhibits nerve growth factor (NGF) withdrawal-induced death in SCG neurons, with an EC50 of ~20 μM. AEG3482 (1-80 μM; 40 h) inhibits p75NTR- and NRAGE- mediated apoptosis of PC12 cells in a dose-dependent manner. AEG3482 (10-40 μM; 30 h) inhibits p75NTR- and NRAGE-mediated JNK activation in PC12 cells. Apoptosis Analysis Cell line: PC12 cells. Concentration: 1, 2.5, 5, 10, 20, 40, 80 μM Incubation time: 40 hours. Result: Reduced p75NTR- or NRAGE-induced cell death by greater than 90% at the concentration of 40 μM. Exerted a slight toxic effect in cells infected with the LacZ control at the concentration of 80 μM. Western blot analysis. Cell line: PC12 cells. Concentration: 10, 20, 40 μM. Incubation time: 30 hours. Result: Attenuated p75NTR- and NRAGE-induced c-Jun phosphorylation and caspase-3 cleavage, and the levels of c-Jun protein.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Apoptosis