ADRB2 Antibody (S364)

SKU: orb1929417

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100, FC - 1:25
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD38482
• Immunogen: This ADRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-373 amino acids from human ADRB2. Antigen Region: 345-373 aa.
• Target: ADRB2
• Molecular Weight: 46459 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, Beta-2 adrenoceptor, ADRB2, ADRB2R, B2AR

Immunohistochemical analysis on paraffin-embedded Human hepato carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Overlay histogram showing A431 cells stained (green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

All lanes: Anti-ADRB2 Antibody (S364) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: HUVEC whole cell lysate. Lane 4: Human liver lysate. Lane 5: NCI-H460 whole cell lysate. Lane 6: PC-3 whole cell lysate. Lane 7: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

ADRB2

UniProt

P07550

RefSeq (Protein):NP_000015.1