ADRA2B Antibody (Center)

SKU: orb1928430

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB22486
• Immunogen: This ADRA2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-369 amino acids from the Central region of human ADRA2B. Antigen Region: 343-369 aa.
• Target: ADRA2B
• Molecular Weight: 49954 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha-2B adrenergic receptor, Alpha-2 adrenergic receptor subtype C2, Alpha-2B adrenoreceptor, Alpha-2B adrenoceptor, Alpha-2BAR, ADRA2B, ADRA2L1, ADRA2RL1

Confocal immunofluorescent analysis of ADRA2B Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of MDA-231 cells using ADRA2B Antibody (Center) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma with ADRA2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-ADRA2B Antibody (Center) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: MDA-MB-231 whole cell lysate. Lane 3: SK-BR-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

ADRA2B

UniProt

P18089

RefSeq (Protein):NP_000673.2