ADRA2B Antibody (Center)

SKU: orb1928430

Description

Research Area

Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	WB - 1:1000
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	49954 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ADRA2L1, ADRA2RL1

Quality Guarantee

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Confocal immunofluorescent analysis of ADRA2B Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of MDA-231 cells using ADRA2B Antibody (Center) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma with ADRA2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-ADRA2B Antibody (Center) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: MDA-MB-231 whole cell lysate. Lane 3: SK-BR-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.