ADK Antibody (N-term)

• Catalog Number: orb1925549
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1103CT3.4.3
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,k
• Dilution range: WB: 1:4000, IHC-P: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 40545 Da
• Target: This ADK antibody is generated from a mouse immunized with a recombinant protein between 1-345 amino acids from human ADK.
• UniProt ID: P55263
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Adenosine kinase, AK, 2.7.1.20, Adenosine 5'-phosp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-ADK Antibody (N-term) at 1:4000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: HL-60 whole cell lysate. Lane 3: Human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining ADK in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.