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Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2

Catalog Number: orb606688

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606688
CategoryAntibodies
DescriptionRecognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.
Species/HostRabbit
ClonalityRecombinant
Clone NumberADFP/2755R
Tested applicationsELISA, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG, kappa
ImmunogenA portion of amin acids 249-376 from the human protein were used as the immunogen for this recombinant Adipophilin antibody.
Antibody TypePrimary Antibody
Dilution rangeELISA (order BSA/sodium azide-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein A affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis recombinant Adipophilin antibody is available for research use only.
UniProt IDQ99541
StorageThe recombinant Adipophilin antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesTitering of the recombinant Adipophilin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2

IHC testing of FFPE human adrenal tissue with recombinant Adipophilin antibody (clone ADFP/2755R). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.

Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2

Western blot testing of human liver lysate with recombinant Adipophilin antibody. Predicted molecular weight ~48 kDa.

Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2

SDS-PAGE analysis of purified, BSA-free recombinant Adipophilin antibody (clone ADFP/2755R) as confirmation of integrity and purity.

  • Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2 [orb639593]

    ELISA,  FACS,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2 [orb2643130]

    ELISA,  FACS,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2 [orb2641441]

    ELISA,  IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    100 μg
  • Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2 [orb534268]

    IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg
  • Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2 [orb639594]

    ELISA,  IHC-P

    Human

    Mouse

    Recombinant

    Unconjugated

    20 μg