Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2

• Catalog Number: orb534268
• Category: Antibodies
• Description: The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [RefSeq]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ADPN1-2R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amin acids 249-376 from the human protein were used as the immunogen for this recombinant Perilipin 2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Prediluted IHC only format: incubate for 30 min at RT (1),Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Perilipin 2 antibody is available for research use only.
• UniProt ID: Q99541
• Storage: The recombinant Perilipin 2 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the recombinant Perilipin 2 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human liver lysate with recombinant Perilipin 2 antibody (clone ADPN1-2R). Predicted molecular weight ~48 kDa.

IHC testing of FFPE human adrenal tissue with recombinant Perilipin 2 antibody (clone ADPN1-2R). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.