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Description
Research Area
Cancer Biology, Cardiovascular Research, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD45438 |
| Immunogen | Synthetic Peptide |
| Target | PLIN2 |
| Molecular Weight | 48075 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-Adipose differentiation-related protein antibody, anti-ADRP antibody, anti-Adipophilin antibody, anti-Perilipin-2, ADFP antibody, anti-PLIN2 antibody
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
ADFP Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-ADFP Antibody (Center) at 1:2000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
ADFP Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADFP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001113.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
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Shu-Hsien Wang 1, Po-Min Chiang 2, Yung-Yeh Su 3 4, Yu-Ting Yu 5 6, Ya-Ping Chen 7, Tsai-Yun Chen 7, L Jeffrey Medeiros 8, Chang-Yao Chu 9 10, Peng-Chieh Chen 2, Kung-Chao Chang Cytoplasmic Lipid Droplets Predict Worse Prognosis in Diffuse Large B-Cell Lymphoma: Next-Generation Sequencing Deciphering Lipogenic Genes Am J Surg Pathol, (2024)
Applications
IHC
Reactivity
Human
Bersuker, Kirill et al. A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes Dev Cell, 44, 97-112.e7 (2018)
ADFP Antibody (Center) (orb35415)
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