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Catalog Number | orb1926280 |
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Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB52740 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500, WB: 1:1000, IHC-P: 1:100, IHC-P: 1:100, IHC-P: 1:100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 121127 Da |
Target | This ADAMTS17 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 508-540 amino acids from the Central region of human ADAMTS17. |
UniProt ID | Q8TE56 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | A disintegrin and metalloproteinase with thrombosp Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-ADAMTS17 Antibody (Center) at 1:1000 dilution + A2058 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 121 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis on paraffin-embedded Human lung tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
All lanes: Anti-ADAMTS17 Antibody (Center) at 1:500 dilution. Lane 1: A431 whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: SK-BR-3 whole cell lysate. Lane 4: RPMI 8226 whole cell lysate. Lane 5: MOLT-4 whole cell lysate. Lane 6: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 121 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.