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ADAM19 Antibody

SKU: orb1248262

Description

ADAM19 Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Porcine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in Human Spleen lysates (calculated MW of 101kDa according to NP_150377.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: KIQCQSSEARPLESN
TargetADAM19
Molecular WeightApprox 125kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ADAM19, ADAM metallopeptidase domain 19, MADDAM, MLTNB, ADAM 19, a disintegrin and metalloproteinase domain 19 (meltrin beta), disintegrin and metalloproteinase domain-containing protein 19, meltrin beta, meltrin-beta, metalloprotease and disintegrin dendritic antigen marker

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ADAM19 Antibody

orb1248262 (0.1 ug/ml) staining of Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ADAM19 Antibody

orb1248262 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ADAM19 Antibody (orb1248262)

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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