ADA Antibody (C-term)

SKU: orb1938050

Description

ADA Antibody (C-term)

Research Area

Cancer Biology, Metabolism Research

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: FC - 1:25, WB - 1:1000
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 40764 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

ADA1

ADA Antibody (C-term) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the ADA antibody detected the ADA protein (arrow).

ADA Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-ADA Antibody (C-term) at 1:1000 dilution. Lane 1: MOLT-4 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HL-60 whole cell lysate. Lane 5: DU145 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human duodenum tissue performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Overlay histogram showing Jurkat cells (green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

UniProt

P00813