Muscle Actin Antibody (Skeletal/Cardiac/Smooth)

SKU: orb2637318

Description

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Muscle Actin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rMSA/953
• Immunogen: Recombinant protein was used as the immunogen for the Muscle Actin antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human skeletal muscle tissue lysate using Muscle Actin antibody (clone rMSA/953). Predicted molecular weight ~45 kDa.

SDS-PAGE analysis of purified, BSA-free Muscle Actin antibody (clone rMSA/953) as confirmation of integrity and purity.

Quick Database Links

UniProt

P68032, P62736, P68133