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ACTG1 Antibody (Center)
Description
Images & Validation
−| Tested Applications | FC, IF, WB |
|---|---|
| Dilution range | WB - 1:1000, FC - 1:10-50, IF - 1:10-50 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, C. elegans, Drosophila, Gallus, Hamster, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus, Yeast, Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 41793 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
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Flow cytometric analysis of K562 cells using ACTG1 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ACTG1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of ACTG1 Antibody (Center) in K562, HepG2 cell line lysates (35 ug/lane). ACTG1 (arrow) was detected using the purified Pab.

Western blot analysis of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.
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Protocol Information
ACTG1 Antibody (Center) (orb1930009)
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