ACTA2/Smooth Muscle Actin Antibody

SKU: orb1537812

Description

ACTA2/Smooth Muscle Actin Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IHC, IHC-P
• Dilution Range: IHC, IHC-P (5 µg/ml)
• Reactivity: Bovine, Gallus, Human, Mouse, Rabbit, Rat
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Positive control: Smooth muscle of arteries, gut wall, uterus and leiomyosarcoma.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin. Cellular Localization: Cytoplasmic
• Target: ACTA2 / Smooth Muscle Actin
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 10 mM PBS, pH 7.6, 15 mM Sodium Azide, 0.2% rAlbumin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACTA2, ACTVS, Alpha-actin-2, Alpha-cardiac actin, AAT6, Actin, aortic smooth muscle, ACTSA, Smooth Muscle Actin, MYMY5, SMA

Quick Database Links

Gene Symbol

ACTA2 / Smooth Muscle Actin