ACTA1/Alpha-actin Antibody (C-term)

SKU: orb1935498

Description

ACTA1/Alpha-actin Antibody (C-term)

Research Area

Cancer Biology, Cardiovascular Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Gallus, Porcine, Rabbit, Xenopus

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 42051 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACTA

Confocal immunofluorescent analysis of ACTA1/α-actin Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

ACTA1/α-actin Antibody (C-term) western blot analysis in A549, RD cell line and human placenta lysates (35 ug/lane). This demonstrates the ACTA1/α-actin antibody detected the ACTA1/α-actin protein (arrow).

ACTA1/α-actin Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ACTA1/α-actin Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ACTA1/α-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

UniProt

P68133