ACSS2 Rabbit pAb Discontinued

SKU: orb247459

Description

Rabbit polyclonal antibody to ACSS2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:10-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 462-701 of human ACSS2 (NP_061147.1).
• Molecular Weight: 78kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ACS antibody, anti ACSA antibody, anti ACAS2 antibody, anti ACECS antibody, anti dJ1161H23.1 antibody

Confocal immunofluorescence analysis of NIH-3T3 cells using ACSS2 Rabbit pAb (orb247459) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using ACSS2 Rabbit pAb (orb247459) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using ACSS2 Rabbit pAb (orb247459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Quick Database Links

UniProt

Q9NR19

Yu Cao 1, Yang Zhao 1, Tan Deng 1, Qigang Zhou 2, Gang Hu 2, Zhuang-Li Hu 3, Yan-Yi Jiang 1, Xiao-Han Yang 1, Fang Wang 4, Peng-Fei Wu 5, Jian-Guo Chen 6 Hepatic acetyl-CoA metabolism modulates neuroinflammation and depression susceptibility via acetate Cell Metab, (2025)

PubMed: 40992374