ACPP Antibody (C-term)

SKU: orb1929125

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:500, IHC-P - 1:10-50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB15146
• Immunogen: This ACPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-341 amino acids from the C-terminal region of human ACPP. Antigen Region: 313-341 aa.
• Target: ACP3 (HGNC:125)
• Molecular Weight: 44566 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Prostatic acid phosphatase, PAP, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase, PAPf39, ACPP

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACPP antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-ACPP Antibody (C-term) at 1:500 dilution. Lane 1: PC-3 whole cell lysate. Lane 2: DU145 whole cell lysate. Lane 3: U-251MG whole cell lysate. Lane 4: Mouse lung whole tissue lysate. Lane 5: Mouse prostate whole tissue lysate. Lane 5: Rat prostate whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

ACP3 (HGNC:125)

UniProt

P15309

RefSeq (Protein):NP_001127666.1, NP_001090.2