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| Catalog Number | orb764459 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to ACOT4 |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 48 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-ACOT4 antibody, anti-PTE2B antibody, anti-PTE Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using ACOT4 Polyclonal Antibody diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunofluorescence analysis of MCF7 cells, using ACOT4 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa and COLO cells, using ACOT4 Antibody. The lane on the right is blocked with the synthesized peptide.
