ACOT1 rabbit pAb

• Catalog Number: orb770027
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ACOT1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human ACOT1. AA range:91-140
• MW: 46
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-ACOT1 antibody, anti-CTE1 antibody, anti-Acyl
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of MCF7 cells, using ACOT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using ACOT1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HT-29 cells using ACOT1 antibody.