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| Catalog Number | orb770027 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to ACOT1 |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACOT1. AA range:91-140 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 46 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-ACOT1 antibody, anti-CTE1 antibody, anti-Acyl Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunofluorescence analysis of MCF7 cells, using ACOT1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using ACOT1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HT-29 cells using ACOT1 antibody.
