ACO2 Antibody

• Catalog Number: orb1249162
• Category: Antibodies
• Description: ACO2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-QDTYQHPPKDSSGQH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 90kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ACO2
• UniProt ID: Q99798
• NCBI: NP_001089.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ACO2, aconitase 2, mitochondrial, ACONM, MGC20605,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 90kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249162 (0.01 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249162 (3.8 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249162 (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Adipose lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249162 (0.01 ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.