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ACK1 Antibody (N-term)
SKU: orb38403
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Description
Research Area
Cancer Biology, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB15601 |
| Immunogen | Synthetic Peptide |
| Target | TNK2 |
| Molecular Weight | 114569 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−anti-Acetate kinase 1 antibody, anti- Acetokinase 1 antibody, anti- ACK 1 antibody, anti- ACK antibody, anti- ACK-1 antibody, anti- ACK1 antibody, anti- ACK1_HUMAN antibody, anti- Activated Cdc42 associated kinase 1 antibody, anti- Activated CDC42 kinase 1 antibody, anti- Activated p21cdc42Hs kinase antibody, anti- FLJ44758 antibody, anti- FLJ45547 antibody, anti- p21cdc42Hs antibody, anti- TNK 2 antibody, anti- TNK2 antibody, anti- Tyrosine kinase non receptor 2 antibody, anti- Tyrosine kinase non receptor protein 2 antibody, anti- Tyrosine kinase non-receptor protein 2 antibody
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Western blot analysis of ACK1 Antibody (N-term) in K562 cell line lysates (35 ug/lane). ACK1 (arrow) was detected using the purified Pab.
Flow cytometric analysis of NCI-H292 cells using ACK1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).
Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with ACK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001010938.1, NP_005772.3 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ACK1 Antibody (N-term) (orb38403)
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