Acetylated Lysine Antibody: HRP

• Catalog Number: orb67533
• Category: Antibodies
• Description: Rabbit polyclonal to Acetylated Lysine(HRP). Detects proteins containing acetylated lysine residues in SDS PAGE immunoblots.
• Target: Acetylated Lysine
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: HRP
• Reactivity: All
• Concentration: 0.25 mg/ml
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Purification: Protein A Purified
• Immunogen: Acetylated KLH Conjugated
• Tested applications: ELISA, IHC, WB
• Dilution range: WB (1:250), ICC/IF (1:100)
• Application notes: A 1/250 dilution of SPC-157 was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: lysine antibody, acetyl lysine antibody
• Research Area: Protein Biochemistry, Signal Transduction
• Note: For research use only

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000.