Acetyl-Histone H4 (Lys5/8/12/16) Recombinant Mouse Monoclonal Antibody

SKU: orb1172811

Description

Acetyl-Histone H4 (Lys5/8/12/16) Recombinant Mouse Monoclonal Antibody

Research Area

DNA and RNA, Epigenetics

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-100, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: C12E6
• Target: H4C1
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Trichostatin A (2μM, 4hr), Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 11 kDa, Observed MW: 11 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, N2a, BRL, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 11 kDa, Observed MW: 11 kDa.

Tissue: Human neuroblastoma, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:500, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172811.

Quick Database Links

Gene Symbol

H4C1