H4K8ac Antibody

SKU: orb1256500

Description

H4K8ac Antibody

Images & Validation

• Tested Applications: ChIP, IF, IHC, IP, WB
• Reactivity: Mouse, Rat
• Application Notes:
  WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:20 - 1:50CHIP seq: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide of human Acetyl-Histone H4-K8
• Target: H4K8ac
• Molecular Weight: Observed: 11kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody (orb1256500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat ovary using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 antibody (orb1256500). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H4K8ac antibody (orb1256500) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Quick Database Links

Gene Symbol

H4K8ac

UniProt

P62805