Acetyl-Histone H3-K9 pAb

• Catalog Number: orb1256503
• Category: Antibodies
• Description: Acetyl-Histone H3-K9 pAb
• Target: H3K9ac
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic peptide of human Acetyl-Histone H3-K9
• UniProt ID: Q16695
• MW: Observed: 16kDa
• Tested applications: ChIP, IF, IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Note: For research use only

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (orb1256503) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (orb1256503) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (orb1256503) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody (orb1256503) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (orb1256503) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 antibody (orb1256503) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours (left). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 antibody (orb1256503) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours (left). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H3K9ac antibody (orb1256503) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.