H3K18ac Antibody

SKU: orb1256501

Description

H3K18ac Antibody

Images & Validation

• Tested Applications: ChIP, IF, IHC, IP, WB
• Reactivity: Mouse, Rat
• Application Notes:
  WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:50 - 1:100CHIP seq: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K18 of human H3
• Target: H3K18ac
• Molecular Weight: Observed: 16kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K18 antibody (orb1256501) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (orb1256501) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat ovary using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded human stomach using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

H3K18ac

UniProt

P68431