Acetyl-Histone H3-K18 pAb

• Catalog Number: orb1256501
• Category: Antibodies
• Description: Acetyl-Histone H3-K18 pAb
• Target: H3K18ac
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K18 of human H3
• UniProt ID: P68431
• MW: Observed: 16kDa
• Tested applications: ChIP, IF, IHC, IP, WB
• Application notes: WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:50 - 1:100CHIP seq: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K18 antibody (orb1256501) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (orb1256501) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat ovary using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded human stomach using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.