H2B K5ac Antibody

SKU: orb1259421

Description

H2B K5ac Antibody

Images & Validation

• Tested Applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100ChIP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K5 of human Histone H2B
• Target: H2B K5ac
• Molecular Weight: Observed: 16kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K5 antibody (orb1259421) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 antibody (orb1259421) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 antibody (orb1259421) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 antibody (orb1259421) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

H2B K5ac