Acetyl-Histone H2B-K12 pAb

• Catalog Number: orb1259423
• Category: Antibodies
• Description: Acetyl-Histone H2B-K12 pAb
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 14kDa
• Target: H2B K12ac
• UniProt ID: Q16778
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: H2B, H2BQ, GL105, H2B.1, H2BFQ, H2BGL105
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody (orb1259423) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. C2C12 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H2B-K12 antibody (orb1259423) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 antibody (orb1259423) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 antibody (orb1259423) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 antibody (orb1259423) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 antibody (orb1259423) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody (orb1259423) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.