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Catalog Number | orb1240300 |
---|---|
Category | Antibodies |
Description | ACE2 Antibody (HRP) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC |
Predicted Reactivity | Bovine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Anti-ACE2 antibody (3217) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2. |
Concentration | 1 mg/mL |
Dilution range | IHC: 2 μg/mL Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested |
Form/Appearance | Liquid |
Conjugation | HRP |
MW | Predicted: 93kDObserved: 130 kD (7 N-linked glycosylation) |
Target | ACE2 |
UniProt ID | Q9BYF1 |
NCBI | NP_068576 |
Storage | ACE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | ACE2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | ACE2 Antibody: ACEH, Angiotensin-converting enzyme Read more... |
Note | For research use only |
Application notes | IHC: 2 μg/mL Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested |
Expiration Date | 12 months from date of receipt. |
ELISA Validation. Coating Antigen: immunogen peptide, orb1236704, 10 ug/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1240300 or orb1239144, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1239144 was detected by anti-rabbit HRP-conjugated secondary antibodies at 1:10000, incubate at RT for 1 hr.
Immunohistochemistry Validation of ACE-2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti- ACE2 antibody (orb1240300, 5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. Counter stained with Hematoxylin.
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