ACE2 Antibody (Biotin)

SKU: orb1240301

Description

ACE2 Antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  IHC: 2 μg/mL Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-ACE2 antibody (biotin) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2.
• Target: ACE2
• Molecular Weight: Predicted: 93kDObserved: 130 kD (7 N-linked glycosylation)
• Purification: ACE2 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor

ELISA Validation. Coating Antigen: immunogen peptide, orb1236704, 10 ug/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1240301 or orb1239144, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1240301 was detected by HRP-conjugated streptavidin at 1:5000 and orb1239144 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10000, incubate at RT for 1 hr.

Immunohistochemistry Validation of ACE-2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti- ACE2 antibody (orb1240301, 5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C, following by streptavidin-HRP conjugate at 20 ug/mL. Counter stained with Hematoxylin.

Quick Database Links

Gene Symbol

ACE2

UniProt

Q9BYF1

RefSeq (Protein):NP_068576