ACE2 Antibody (Biotin)

• Catalog Number: orb1240301
• Category: Antibodies
• Description: ACE2 Antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-ACE2 antibody (biotin) (3217) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2.
• Concentration: 1 mg/mL
• Dilution range: IHC: 2 μg/mL Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Biotin
• MW: Predicted: 93kDObserved: 130 kD (7 N-linked glycosylation)
• Target: ACE2
• UniProt ID: Q9BYF1
• NCBI: NP_068576
• Storage: ACE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ACE2 Antibody: ACEH, Angiotensin-converting enzyme
• Note: For research use only
• Application notes: IHC: 2 μg/mL Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

ELISA Validation. Coating Antigen: immunogen peptide, orb1236704, 10 ug/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1240301 or orb1239144, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1240301 was detected by HRP-conjugated streptavidin at 1:5000 and orb1239144 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10000, incubate at RT for 1 hr.

Immunohistochemistry Validation of ACE-2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti- ACE2 antibody (orb1240301, 5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C, following by streptavidin-HRP conjugate at 20 ug/mL. Counter stained with Hematoxylin.