ACAT1 Antibody

• Catalog Number: orb1255472
• Category: Antibodies
• Description: ACAT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 42kDa
• Target: ACAT1
• UniProt ID: P24752
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, AC
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ACAT1 antibody (orb1255472) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human lung cancer using ACAT1 antibody (orb1255472) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using ACAT1 antibody (orb1255472) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using ACAT1 antibody (orb1255472) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using ACAT1 antibody (orb1255472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using ACAT1 antibody (orb1255472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using ACAT1 antibody (orb1255472) at dilution of 1:100. Blue: DAPI for nuclear staining.