Acarbose

SKU: orb1310795

Description

Acarbose

Research Area

Metabolism Research

Key Properties

• CAS Number: 56180-94-0
• MW: 645.60
• Purity: 99.89%
• Formula: C₂₅H₄₃NO₁₈
• SMILES: O([C@@H]1[C@@H](CO)O[C@H](O[C@@H]([C@@H]([C@H](C=O)O)O)[C@@H](CO)O)[C@H](O)[C@H]1O)[C@@H]2[C@H](O)[C@@H](O)[C@H](N[C@H]3C=C(CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H](C)O2
• Target: glycosidase,Glucosidase
• Solubility: H2O:64.5 mg/mL (99.91 mM);DMSO:50 mg/mL (77.45 mM)

Bioactivity

• Target IC50:
  maltose (1 g/l):36 μg/ml|Caco-2 cells:5 μM|α-glucosidase:2.154 µg/mL|maltose (2 g/l):57 μg/ml|sucrose (4 g/l):3.2 μg/ml|α-glucosidase:11 nM
• In Vivo:
  METHODS: To study the hypoglycemic effect of Acarbose, rats were treated with Acarbose (30, 60 mg/kg) by gavage once daily for 8 weeks. RESULTS: Acarbose reduced fasting glucose and modulated glucose tolerance without weight loss in DM rats. Acarbose significantly inhibited serum IL6 and TNF-α in DM rats. METHODS: To study the effect of Acarbose on atherosclerosis, rabbits were fed Acarbose (2.5-5.0 mg/kg) for 25 weeks. RESULTS: Acarbose significantly and dose-dependent reduced the intensity of IL-6, TNF-α and iNOS staining, and significantly increased the intensity of p-AMPK staining in the neointima. Acarbose significantly and dose-dependent reduced neointimal Ras and β-galactosidase expression in HCD-fed rabbits without weight loss.
• In Vitro:
  METHODS: A7r5 cells pretreated with TNF-α were treated with Acarbose (1, 2, and 3 μM) and the expression levels of target proteins were detected by Western Blot. RESULTS: Acarbose decreased the expression of β-galactosidase and Ras and increased the expression of p-AMPK in a dose-dependent manner.
• Cell Research:
  Cell viability is determined using the MTT assay. Cells are seeded in 24-well culture plates at a density of 2×104 cells/well, incubated for 48 h, treated with acarbose at varying concentrations (0.5, 1.0, 2.0, 3.0, and 5.0 μM) for 24 h; or pre-treated with TNF-α (20 ng/mL) for either 24 h or 48 h to evaluate the dose-dependent effects of acarbose on VSMC growth and viability, cultured with 0.5 mg/mL MTT at 37°C in a humidified atmosphere of 5% CO2 for another 4 h, and solubilized with isopropanol. The viable cell number varies directly with the concentration of formazan product measured spectrophotometrically at 563 nm.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BAY g 5421, BAY g5421, BAY g-5421, antihyperglycemic, anti-diabetes, Acarbose, oral, inhibit, mellitus, Inhibitor, insulin, Glucosidase, α-glucosidase

Compound Identifiers

PubChem CID

9811704