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Catalog Number | orb1247853 |
---|---|
Category | Antibodies |
Description | ACAP2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Canine, Porcine |
Reactivity | Human, Mouse, Rat |
Immunogen | The immunogen for this antibody is: C-EKLNRFQQDSQKF |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 90kDa band observed in Mouse and Rat Lung lysates and in lysates of cell lines MOLT4 and NIH3T3 (calculated MW of 88.1kDa according to Human NP_036419 and 87.2kDa according to Mouse NP_084414.1 and Rat NP_001029178.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | ACAP2 |
UniProt ID | Q15057 |
NCBI | NP_036419.3 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | ACAP2, CENTB2, centaurin, beta 2, CNT-B2, KIAA0041 Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 90kDa band observed in Mouse and Rat Lung lysates and in lysates of cell lines MOLT4 and NIH3T3 (calculated MW of 88.1kDa according to Human NP_036419 and 87.2kDa according to Mouse NP_084414.1 and Rat NP_001029178.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247853 (1 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1247853 (5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247853 (0.3 ug/ml) staining of Mouse (A) and Rat (B) Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1247853 (0.3 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, IHC, WB | |
Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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