ACAP2 Antibody

• Catalog Number: orb1247853
• Category: Antibodies
• Description: ACAP2 Antibody
• Target: ACAP2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-EKLNRFQQDSQKF
• UniProt ID: Q15057
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 90kDa band observed in Mouse and Rat Lung lysates and in lysates of cell lines MOLT4 and NIH3T3 (calculated MW of 88.1kDa according to Human NP_036419 and 87.2kDa according to Mouse NP_084414.1 and Rat NP_001029178.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ACAP2, CENTB2, centaurin, beta 2, CNT-B2, KIAA0041
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_036419.3

orb1247853 (1 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247853 (5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247853 (0.3 ug/ml) staining of Mouse (A) and Rat (B) Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247853 (0.3 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.