ACADM Antibody (Center)

SKU: orb1929779

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB20911
• Immunogen: This ACADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the Central region of human ACADM. Antigen Region: 189-217 aa.
• Target: ACADM (HGNC:89)
• Molecular Weight: 46588 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Medium-chain specific acyl-CoA dehydrogenase, mitochondrial, MCAD, ACADM

Flow cytometric analysis of widr cells using ACADM Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ACADM Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACADM Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of ACADM Antibody (Center) in mouse heart tissue lysates (35 ug/lane). ACADM (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

ACADM (HGNC:89)

UniProt

P11310

RefSeq (Protein):NP_001120800.1, NP_000007.1