ACACA Antibody

• Catalog Number: orb1259430
• Category: Antibodies
• Description: ACACA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human ACACA
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 240kDa
• Target: ACACA
• UniProt ID: Q13085
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Acetyl-CoA carboxylase 1, ACC1, ACC-alpha, Biotin
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ACACA antibody (orb1259430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of 293T cells using ACACA antibody (orb1259430) at dilution of 1:100. 293T cells were treated by Hydrogen Peroxide (2 nM) at 37 ℃ for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using ACACA antibody (orb1259430) at dilution of 1:100. C6 cells were treated by Hydrogen Peroxide (2 nM) at 37 ℃ for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using ACACA antibody (orb1259430) at dilution of 1:100. HeLa cells were treated by Hydrogen Peroxide (2 nM) at 37 ℃ for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.