Ac-FLTD-CMK

SKU: orb1693416

Description

Ac-FLTD-CMK is a cell-permeable, irreversible inhibitor derived from abscisicin D that selectively targets inflammatory caspases. It potently inhibits caspase-1, -4, and -11 (with IC50 values in the nM to µM range) but not caspase-3, making it a useful tool for studying pyroptosis and inflammasome biology in cellular and animal models of inflammation.

Research Area

Cell Biology, Immunology & Inflammation, Protein Biochemistry

Key Properties

• CAS Number: 2376255-48-8
• MW: 569.05
• Purity: 98.61% (May vary between batches)
• Formula: C₂₆H₃₇ClN₄O₈
• SMILES: CC(C)C[C@H](NC(=O)[C@H](Cc1ccccc1)NC(C)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)CCl
• Target: Pyroptosis,Caspase
• Solubility: DMSO:150 mg/mL (263.6 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (5.8 mM)

Bioactivity

• Target IC50:
  Caspase-5:329 nM (IC50)|Caspase-1:46.7 nM (IC50)|Caspase-4:1.49 μM (IC50)
• In Vitro:
  Ac-FLTD-CMK, inhibits GSDMD cleavage by caspases-1, -4, -5, and -11 in vitro, suppresses pyroptosis downstream of both canonical and noncanonical inflammasomes, as well as reduces IL-1β release following activation of the NLRP3 inflammasome in macrophages. By contrast, Ac-FLTD-CMK does not target caspase-3 or apoptotic cell death. Crystal structure of caspase-1 in complex with Ac-FLTD-CMK reveals extensive enzyme-inhibitor interactions involving both hydrogen bonds and hydrophobic contacts.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

caspase-4, caspase-1, caspase-11, Ac FLTD CMK, AcFLTDCMK, Ac-FLTD-CMK

Compound Identifiers

PubChem CID

155906572