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| Catalog Number | orb1249542 |
|---|---|
| Category | Antibodies |
| Description | ABTB1 Antibody |
| Target | ABTB1 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Canine, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 500 ug/mL |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | The immunogen for this antibody is: RALEDLLVSIGLDC |
| UniProt ID | Q969K4 |
| Tested applications | ELISA, IHC, WB |
| Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in Human Heart lysates (calculated MW of 37.6kDa according to NP_115937.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Heart and Brain (Cerebellum). Recommended concentration: 3.75ug/ml. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | ABTB1, BPOZ, EF1ABP, MGC20585, ankyrin repeat and Read more... |
| Research Area | Cancer Biology |
| Note | For research use only |
| NCBI | NP_742025.1, NP_115937.1, NP_742024.1 |

orb1249542 staining (1 ug/ml) of Human Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1249542 (3.8 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Canine, Mouse, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Equine, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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