ABTB1 Antibody

• Catalog Number: orb1249542
• Category: Antibodies
• Description: ABTB1 Antibody
• Target: ABTB1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: RALEDLLVSIGLDC
• UniProt ID: Q969K4
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in Human Heart lysates (calculated MW of 37.6kDa according to NP_115937.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Heart and Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ABTB1, BPOZ, EF1ABP, MGC20585, ankyrin repeat and
• Note: For research use only
• NCBI: NP_115937.1, NP_742025.1, NP_742024.1

orb1249542 staining (1 ug/ml) of Human Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1249542 (3.8 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.