ABO Antibody (Blood Group Antigen A)

• Catalog Number: orb749540
• Category: Antibodies
• Description: This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgM, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A mixture of erythrocytes of blood group A and glycoprotein fraction isolated from the saliva of secretors with blood group A was used as the immunogen for the ABO antibody.
• UniProt ID: P16442
• Tested applications: IF, IH
• Dilution range: Immunofluorescence: 2-4ug/ml,Immunohistology (formalin-fixed): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the ABO antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Antibody Type: Primary Antibody
• Clone Number: HE-193
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This ABO antibody is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Antigen A antibody (HE-193)