ABO Antibody (Blood Group Antigen H Type 2)

• Catalog Number: orb749538
• Category: Antibodies
• Description: Recognizes the blood group H type 2 antigens, trisaccharide Fuc(a1-2)Gal(b1-4)GlcNAc(b1) of human origin. This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgM, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Mucinous colonic adenocarcinoma was used as the immunogen for the ABO antibody.
• UniProt ID: P16442
• Tested applications: IF, IHC-P
• Dilution range: Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Optimal dilution of the ABO antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Antibody Type: Primary Antibody
• Clone Number: 19-OLE
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This ABO antibody is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Antigen H Type 2 antibody (19-OLE)